macでtmuxを使いはじめました。

しかし Ctrl + a and Shift + ¥(|)

としてもPaneが分割されません、どうしてですか？

set-option -g prefix C-a
bind-key C-a send-prefix

# split panes using | and -
bind | split-window -h
bind - split-window -v
unbind '"'
unbind %

# force a reload of the config file
# reload config file (change file location to your the tmux.conf you want to use)
bind r source-file ~/.tmux.conf

# quick pane cycling
unbind ^A
bind ^A select-pane -t :.+

# smart pane switching with awareness of vim splits
bind -n C-h run "(tmux display-message -p '#{pane_current_command}' | grep -iq vim && tmux send-keys C-h) || tmux select-pane -L"
bind -n C-j run "(tmux display-message -p '#{pane_current_command}' | grep -iq vim && tmux send-keys C-j) || tmux select-pane -D"
bind -n C-k run "(tmux display-message -p '#{pane_current_command}' | grep -iq vim && tmux send-keys C-k) || tmux select-pane -U"
bind -n C-l run "(tmux display-message -p '#{pane_current_command}' | grep -iq vim && tmux send-keys C-l) || tmux select-pane -R"
bind -n C-\ run "(tmux display-message -p '#{pane_current_command}' | grep -iq vim && tmux send-keys 'C-\\') || tmux select-pane -l"

# resize pane
bind -n C-Down resize-pane -D 5
bind -n C-Up resize-pane -U 5
bind -n C-Right resize-pane -R 10
bind -n C-Left resize-pane -L 10

bind -n S-Down resize-pane -D 1
bind -n S-Up resize-pane -U 1
bind -n S-Right resize-pane -R 2
bind -n S-Left resize-pane -L 2
# tmux status bar color
set-option -g status-keys "emacs"
set -g status-left-length 50
set -g status-right " Show Terminal #(date '+%a, %b %d - %I:%M') "

bind c new-window -c "#{pane_current_path}"

set -g renumber-windows on

bind-key b break-pane -d
bind-key C-j choose-tree

# Use vim keybindings in copy mode
setw -g mode-keys vi

# Setup 'v' to begin selection as in Vim
bind-key -t vi-copy v begin-selection
bind-key -t vi-copy y copy-pipe "reattach-to-user-namespace pbcopy"

# Update default binding of `Enter` to also use copy-pipe
unbind -t vi-copy Enter
bind-key -t vi-copy Enter copy-pipe "reattach-to-user-namespace pbcopy"